Investigators wanted to analyze the diagnostic performance of methylation-sensitive restriction enzyme digestion followed by sequencing using cell-free DNA and explore the cancer signal origin of the ...

Scientists first observed the biological phenomenon of restriction and modification in the 1950s, but they did not identify and isolate restriction enzymes until the late 1960s. 3 In 1970, Hamilton ...

Enzymes that cut DNA at specific sites are called endonucleases; these enzymes play many roles in genomic replication, fidelity, and defense. The initial discovery of restriction endonucleases in the ...

Cancer, as a disease, starts with a change in the genome that results in the downregulation of genes involved in "normal" cell behavior. This genetic change can either be induced by a change in the ...

The origin of SARS-CoV-2 remains unresolved. In a non-peer-reviewed preprint published on bioRxiv on Oct. 20, 2022 three authors present analyses that, according to their interpretation, suggest a ...

Cambridge, MA - Traditional bisulfite sequencing damages DNA, while enzyme-based alternatives are inconsistent. A novel methylation analysis method, called UMBS-seq, was published this week in Nature ...

DNA can also be demethylated, either through passive or active processes. Passive DNA demethylation occurs when the methylation pattern is not replenished during DNA replication and gradually ...

DNA and RNA methylation, initially thought to operate as distinct pathways, have been shown to interact closely, shifting our understanding of gene regulation. A team of researchers from the ...

To many working biologists, DNA-modifying enzymes are tools, not unlike a pair of molecule-sized scissors. To Dr. Barry Stoddard, a professor in the Basic Sciences division at Fred Hutch, these ...