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Gram staining is one of the most crucial staining techniques in microbiology. The name comes from the Danish bacteriologist Hans Christian Gram, who first introduced it in 1882 to identify organisms causing pneumonia. Typically, Gram staining is the first test performed, utilizing crystal violet or methylene blue as the primary color. Organisms that retain the primary color and appear purple-brown under a microscope are gram-positive. In contrast, those that do not take up the primary stain and
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